These are notes in support of some ideas that I’m working on at The Baltimore UnderGround Science Space.  The first is this thought of developing processes to produce high value chemicals or substances using biotechnology rather than standard chemical processes.  The main technologies to exploit are CRISPR/ Cas9 (or some derivative), cell free technologies and something called SCRaMbLE which introduces some genetic diversity in a way to more efficiently do directed evolutions studies. I will publish some key notes of literature research I am doing on this.  My key concern is that perhaps CRISPR editing could introduce the the same effects as SCRaMbLE efficiently.  Need to understand the advantages of using both.




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